NCG-B2m-KO

Strain Name: NOD/ShiLtJGpt-Prkdc em26Cd52Il2rgem26Cd52B2mem1Cd4/Gpt

Strain Type:Knock-out

Strain ID: T004670

Background: NOD/ShiLtJGpt

Description

NCG mice carry mutations in Prkdc and Il2rg gene in NOD/ShiLtJGpt genetic background. Mutations in the Prkdc gene result in impaired VDJ recombination and the loss of mature T and B cells. The gamma chain of Il2 receptor is a co-receptor subunit of IL-2, IL-4, IL-7, IL-9, IL-15 and IL-21, which are important immune functions, after knocking out the IL2rg gene, the immune function of the mouse is seriously reduced, especially the activity of NK cells is almost lost. B2m, alias Ly-m11, beta2m, beta2-m, β2-microglobulin, is an essential component for the transport of MHC class I proteins to the cell surface. The B2m knockout homozygous mouse cell surface has almost no expression of MHC class I protein, and also have the following characteristics: NK cell deficiency, NK+ T cell deficiency, and decreased serum Ig levels.

GemPharmatech use gene editing technology to knock out the B2m gene of NCG mice, developed NCG-B2m-KO model independently. This strain combines the characteristics of severe immunodeficiency mutation (scid), IL2rγ null and MHC I (beta-2 microglobulin) deficiency, and is highly resistant to graft-versus-host disease (GVHD). A useful model to study the in vivo mechanisms of the heterologous gene GVHD and to rapidly assess therapeutic agents.

Strategy

Strategy of B2m KO mouse

Application

1. Human immune system reconstitution (HSC, PBMC)

2. Human tumor cell/tissue transplantation

4. Human hematopoietic system and immune system research

5. Studies on graft versus host disease (GVHD)

Data Support

MHCI expression level detection

An anti-H2K antibody was used to stain the splenocytes from NCG and NCG-B2m KO mice, 59.3% of CD45+ cells from NCG control mice were H2K+, while splenocytes from NCG-B2m KO mice were H2K-.